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alpha tubulin  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology alpha tubulin
    Alpha Tubulin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clathrin+lc/Clathrin+LC+Antibody/pmc12615670__41467_2025_64948_MOESM2_ESM-73-53-54
    Average 92 stars, based on 14 article reviews
    alpha tubulin - by Bioz Stars, 2026-10
    92/100 stars

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    Incubation:

    Article Title: AT 1 receptor-mediated uptake of angiotensin II and NHE-3 expression in proximal tubule cells through a microtubule-dependent endocytic pathway
    Article Snippet: PT protein samples (10 μg each) were electrophoretically separated on 8–16% Tris-glycine gels at 120 V for 1.5–2 h. After SDS separation, proteins were transferred to Millipore Immobilon-P membranes using a Bio-Rad Trans-Blot Semi-Dry system powered by a Bio-Rad Power-Pac HC (25 V, 0.12 A, 1.5 h). .. The membranes were blotted overnight at 4°C with 5% nonfat dry milk and incubated for 3 h at room temperature with primary antibodies against the human AT 1 receptor (1:200), clathrin LC or HC (1:200), MAP-1A or MAP-1B (1:200, Santa Cruz Biotechnology), NHE-3 proteins (1:200, Chemicon), and t-ERK1/2 and p-ERK1/2 (1:200, Cell Signaling) ( 7 , 25 , 28 ). .. To ensure equal protein loading, the same membranes were treated with stripping buffer (Pierce) for 20 min, blotted with 5% nonfat dry milk, and reprobed with a mouse anti-β-actin monoclonal antibody at 1:2,000 (Sigma-Aldrich).

    Biomarker Discovery:

    Article Title: Heat shock protein DNAJA2 controls insulin signaling and glucose homeostasis by preventing spontaneous insulin receptor endocytosis
    Article Snippet: .. Ethics oversight Insulin Receptor beta (E9L5V) Rabbit mAb (CST #23413) Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151)(19H7) Rabbit mAb (CST #3024) Phospho-Akt (Thr308) (CST #13038) Akt (pan) (CST #4691) GSK-3beta (CST #12456) Phospho-GSK-3beta (Ser9) (CST #5558) Rab7 (CST #9367) p-Akt1 (Santa Cruz sc-81433) beta Adaptin (Santa Cruz sc-74423) Clathrin LC (Santa Cruz sc-12735) alpha-Tubulin (Santa Cruz sc-23948) HSC70 (Santa Cruz sc-7298) HA-tag (Santa Cruz sc-7392) Insulin Receptor beta subunit, clone CT-3 (Millipore Sigma #MABS65) Mouse anti-DNAJA2 mAb (Millipore Sigma #WH0010294M1) Rabbit anti-DNAJA2 (Abcam ab157216) DNAJA2 Polyclonal antibody (Proteintech #12236-1-AP) Na+/K+-ATPase Rabbit mAb (Abclonal A11683) Describe the validation of each primary antibody for the species and application, noting any validation statements on the manufacturer’s website, relevant citations, antibody profiles in online databases, or data provided in the manuscript. .. Human HeLa and mouse 4T1, MC38 cells were obtained from ATCC; HepG2-IR cell was provided by Dr. Choi (Columbia University).



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    LICUS activates the <t>clathrin-mediated</t> pathway. ( A ) Western blot analysis of two important endocytosis pathways, clathrin <t>and</t> <t>caveolin,</t> in HepG2 cells with or without CPZ treatment. The clathrin-mediated pathway was successfully inhibited. ( B ) Uptake of dye-labeled H-Exos into HepG2 cells with or without CPZ treatment monitored by confocal laser scanning microscopy. Cell nuclei were labeled with DAPI (blue) signals, and exosomal membranes were labeled with common exosome marker CD63-Cy3 (red) signals. LICUS-triggered exosome uptake was mediated mainly by the clathrin pathway. Confocal microscopy image scale bar: 10 μm. ** p < 0.01 means comparison with the control group. * p < 0.05 means comparison with the control group. Values are the means ± SD of at least three independent experiments.
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    Image Search Results


    LICUS activates the clathrin-mediated pathway. ( A ) Western blot analysis of two important endocytosis pathways, clathrin and caveolin, in HepG2 cells with or without CPZ treatment. The clathrin-mediated pathway was successfully inhibited. ( B ) Uptake of dye-labeled H-Exos into HepG2 cells with or without CPZ treatment monitored by confocal laser scanning microscopy. Cell nuclei were labeled with DAPI (blue) signals, and exosomal membranes were labeled with common exosome marker CD63-Cy3 (red) signals. LICUS-triggered exosome uptake was mediated mainly by the clathrin pathway. Confocal microscopy image scale bar: 10 μm. ** p < 0.01 means comparison with the control group. * p < 0.05 means comparison with the control group. Values are the means ± SD of at least three independent experiments.

    Journal: Bioengineering

    Article Title: Low-Intensity Continuous Ultrasound Enhances the Therapeutic Efficacy of Curcumin-Encapsulated Exosomes Derived from Hypoxic Liver Cancer Cells via Homotropic Drug Delivery Systems

    doi: 10.3390/bioengineering11121184

    Figure Lengend Snippet: LICUS activates the clathrin-mediated pathway. ( A ) Western blot analysis of two important endocytosis pathways, clathrin and caveolin, in HepG2 cells with or without CPZ treatment. The clathrin-mediated pathway was successfully inhibited. ( B ) Uptake of dye-labeled H-Exos into HepG2 cells with or without CPZ treatment monitored by confocal laser scanning microscopy. Cell nuclei were labeled with DAPI (blue) signals, and exosomal membranes were labeled with common exosome marker CD63-Cy3 (red) signals. LICUS-triggered exosome uptake was mediated mainly by the clathrin pathway. Confocal microscopy image scale bar: 10 μm. ** p < 0.01 means comparison with the control group. * p < 0.05 means comparison with the control group. Values are the means ± SD of at least three independent experiments.

    Article Snippet: Antibodies against HIF-1α, caveolin-1, CD63, TSG101, cytochrome C, calnexin, clathrin, Rab27a, Rab7, β-actin, TIMP3, S100A4, and Bcl-2 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: Western Blot, Labeling, Confocal Laser Scanning Microscopy, Marker, Confocal Microscopy, Comparison, Control